玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1503STO 小鼠胚成纖維細(xì)胞系

STO 小鼠胚成纖維細(xì)胞系

簡要描述:CRL-1503 STO 小鼠胚成纖維細(xì)胞系
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-1503
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2140

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系 的詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系

ATCC® Number:CRL-1503™  Price:$289.00
Designations:STO

Depositors:G Martin

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Organ: embryo
Strain: SIM
Cell Type: fibroblast


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1503

Isolation:Toronto Ontario, Canada

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Age:embryo

Comments:The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto, Canada from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. The cell line is used routinely to prepare feeder layers by irradiation or mitomycin C treatment. Such populations are then employed for maintenance of stock teratocarcinoma stem cells (see ATCC CRL-1535 and CRL-1566) in the undifferentiated state.The cells have been tested and found negative for ectromelia virus (mousepox).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

irradiated to be used as feeder cells:ATCC 56-X



References:1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

21874: . Teratomas and differentiation. New York: Academic Press; 1975.

22387: . . Cell 6: 467-474, 1975.

22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
护士扒下内裤让我爽一夜| 少妇人妻偷人精品无码AV| 国产av精品国语对白国产| 强行扒开双腿玩弄AV调教视频| 乱人伦精品视频在线观看| 浴室人妻的情欲HD三级| 中文字幕人妻无码乱精品| 高清视频在线观看| 国产精品久久久久久亚洲| 久久精品国产亚洲AV无码麻豆| 偷偷鲁2020精品偷拍视频| 国产熟女高潮视频| 精品国产乱码久久久久久1区2区| 日本免费观看| 精品人妻系列无码一区二区三区| 女人下边紧了好还是松点好| 精品人妻无码一区二区三区牛牛 | 亚洲av噜噜一区二区三区| 紫川动漫在线观看免费完整版| 久久99精品久久久久久水蜜桃 | 午夜精品久久久久久久| 51国偷自产一区二区三区| 性色AV浪潮AV色欲AV| 日日躁夜夜躁狠狠久久AV| 欧美一区二区三区成人片在线| 性饥渴老太XXXXXHD| 一本狠狠色丁香婷婷综合久久| 国产欧美日韩精品丝袜高跟鞋| 日本无码精油按摩WWW视频 | 亚洲熟女综合一区二区三区| 同性两个17男互摸互吃的小说| 久久热精品视频| MM131亚洲精品久久安然| 久久这里只有精品| 熟女人妻少妇精品视频| AV无码精品一区二区三区宅噜噜| 精品久久久久| 中文无码成人免费视频在线观看| 国产精品久久久久久麻豆一区 | 永久免费av网站| 国产AV一区二区三区最新精品|