玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12 小鼠成肌細(xì)胞

C2C12 小鼠成肌細(xì)胞

簡(jiǎn)要描述:CRL-1772 C2C12 小鼠成肌細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2226

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞的詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™  Price:$256.00
Designations:C2C12

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:myoblast
C2C12 小鼠成肌細(xì)胞


Source:Strain: C3H
Tissue: muscle
Cell Type: myoblast;


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this . AATCC materialnyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1772

Applications:transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)



Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]

The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]

Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.

Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.

Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

    Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.

  6. Incubate cultures at 37°C.




















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
五月天中文字幕MV在线| 被绑到房间用各种道具调教| 国产精品偷伦视频免费观看了| 绝伦の上司に一晚人妻| 成人H动漫精品一区二区| 黑人巨大粗物挺进了少妇| 中文字幕熟女人妻佐佐木| 久久性爱视频| 丽娟两腿间一大丛黑毛| 超碰CAOPOREN97人人| 精品亚洲A∨一区二区三区| HD女人奶水授乳MILK| 色综合久久精品亚洲国产| 成人精品视频99在线观看免费| 99久久国产露脸国语对白| 粗大黑人巨精大战欧美成人免费看 | 中国A级毛片免费观看| 久久久国产精华液2023| 国产乱国产乱老熟300部| 亚洲18色成人网站WWW| 黑人60厘米全进去了| 久久精品一区二区三区| 亚洲国产精品成人综合色在线| 国产一区二区三区在线观看| 大学生高潮无套内谢视频| 无码播放一区二区三区| 精品国产一区二区三区久久久狼| 国产精品99精品无码视亚| 老师上课没戴奶罩看到奶头| 玩弄白嫩少妇XXXXX性| 欧美多人乱大交XXXXX变态| 日韩吃奶摸下aa片免费观看| 日本XXXB孕妇孕交视频| 啦啦啦资源视频在线观看8| 色情版劳拉的性放荡A片| 国产精品国产三级国产a| 无码精品国产一区二区三区免费| 在线永久免费观看黄网站| 人妻少妇被猛烈进入中文字幕| 久久av无码av高潮av| 免费人成在线观看网站|