玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-148H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

簡(jiǎn)要描述:H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-148
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2024-11-21
  • 訪(fǎng)  問(wèn)  量:1832

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

ATCC® Number:HTB-148™    Price:$338.00
Designations:H4

Depositors:J Riggs

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: brain
Disease: neuroglioma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞.

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 12

D16S539: 11,12

D5S818: 10,12

D7S820: 8,11

THO1: 7,9

TPOX: 8,11

vWA: 14,18



Cytogenetic Analysis:modal number = 73; range = 63 to 78.

This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes.



Isoenzymes:AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1-2

PGM3, 1



Age:37 years

Gender:male

Ethnicity:Caucasian

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:              
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed:4544026
22907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131

















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品久久人妻互换| 久久久国产一区二区三区四区小说 | 国产精品黄在线观看免费软件 | 亚洲av无码一区二区三区在线| 精品人伦一区二区三区蜜桃免费| 国产片xxxxa片国语对白| 护士在办公室里被躁中文字幕 | 国产睡熟迷奷系列精品| 久久天天躁狠狠躁夜夜| 无套和妇女做内谢| 中国人体棚拍室内摄影| 51久久成人国产精品麻豆| 被黑人猛躁10次高潮视频| 男女裸体做爰猛烈全过程免费视频 | xxxx丰满小少妇女自小说| 国产猛男猛女超爽免费视频| 国产精品日韩欧美一区二区三区| 丝袜美腿一区二区三区| 日韩精品无码一区aaa片| 97无码欧美熟妇人妻蜜桃天美 | 日本动漫在线| 太粗太硬小寡妇受不了视频| china贵州少妇video| 国产精品久久久久精品| 国产亚洲精品99一区二区 | 欧美熟妇VIVOE精品| 迎合疯狂肉伦耕耘播种| 欧美性狂猛XXXXX深喉| 白丝JK校花娇喘求饶白浆露出 | 公司领导要了我好几次| 又硬又粗又长又大时间持久| 久久久精品一区二区三区免费| 国产精品亚洲一区二区无码| 日本动漫瀑乳H动漫啪啪免费| 女人洗澡沐浴露全身| 国产理论片午午伦夜理片2021| 天堂在/线资源中文在线| 中国VITAFUSION孕妇| 无码人妻丰满熟妇区免费| 99这里只有精品| 妺妺窝人体色777777野大粗|