玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-148H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

簡(jiǎn)要描述:H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-148
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1612

產(chǎn)品分類

Product Category

詳細(xì)介紹

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

ATCC® Number:HTB-148™    Price:$338.00
Designations:H4Depositors: J RiggsBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: brain Disease: neurogliomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞Applications:transfection host (Roche FuGENE® Transfection Reagents)Tumorigenic:NoDNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,9 TPOX: 8,11 vWA: 14,18Cytogenetic Analysis:modal number = 73; range = 63 to 78.This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 1Age:37 yearsGender:maleEthnicity:CaucasianPropagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:             Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed:454402622907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美jizz18性欧美| 欧洲美女与动性ZOZOZO| 久久无码专区国产精品S| 我和岳M愉情XXXX国产| 精品国产一区二区三区久久| 色婷婷五月综合中文字幕| 我把护士日出水了视频90分钟| 免费毛片在线看不用播放器 | 欧美bbbwbbwbbwbbw| 小婷的性放荡日记H| 久久精品国产亚洲AV香蕉高清| 老师好大好爽我要喷水了视频| 厚颜无耻韩国动漫在线阅读视频| 精品无码久久久久久久久水蜜桃 | 亚洲熟女AV中文字幕男人总站| 久久久久亚洲AV无码专区电影| 人妻出轨系列38部分阅读| 欧美高清videossexo| 国产性生交XXXXX无码| 欧美性大战久久久久久久| 欧美性色黄大片a级毛片视频| 女人自己扒开荫道口视频| 国产精品亚洲一区二区三区在线| 日本熟妇乱妇熟色A片蜜桃| 麻花豆传媒MV在线观看网站| 女上男下啪啪激烈高潮无遮盖| 男妓用舌头舔我高潮不退| 黑人添女人囗交做爰视频| 日韩AV片无码一区二区不卡电影| 日本无码色情影片在线看| 国产400部AV国片免费| 男人一边吃奶一边做爰免费视频| 亚洲日韩一区二区三区四区高清| 亚洲色成人网站WWW永久四虎 | 国产免费视频| 久久自己只精产国品| 性饥渴艳妇K8经典A片| 国产精品无码麻豆放荡AV| 国语熟妇乱人乱A片久久| 色婷婷五月综合中文字幕| 无码精品一区二区三区在线|