玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12細(xì)胞, 小鼠成肌細(xì)胞

C2C12細(xì)胞, 小鼠成肌細(xì)胞

簡(jiǎn)要描述:C2C12細(xì)胞, 小鼠成肌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1929

產(chǎn)品分類

Product Category

詳細(xì)介紹

C2C12細(xì)胞, 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™    Price:$256.00
Designations:C2C12Biosafety Level:1Shipped:frozenMedium Properties:& Serum:oSee PropagationGrowthadherentOrganism:Mus musculus (muse)Morphology:myoblastSource:Strain: C3H Tissue: muscle Cell Type: myoblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. C2C12細(xì)胞, 小鼠成肌細(xì)胞Applications:transfection host (Nucleofection technology from LonzaRoche FuGENE® Transfection Reagents)Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903] The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953] Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] Tested and found negative for ectromelia virus (mousepox).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.Incubate cultures at 37°C.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
少妇做爰特黄A片免费看| 黑人玩弄漂亮少妇高潮大叫| 日本va欧美va精品发布| 偷拍真实夫妇作爱视频| 淑芬两腿间又痒内裤不透气| 我才12因啪啪就破了处怎么办| 小寡妇高潮流白浆A片| 少妇人妻系列1~100| 女子初尝黑人巨嗷嗷叫| 玩50岁四川熟人A片| 我把护士日出水了视频90分钟| 扒开粉嫩小泬舌头伸进去| 人人妻人人爽人人做夜欢视频九色| 精国产品一区二区三区A片| 中文字幕乱码中文| 色婷婷久久啪啪一区二区| 偷窥丶偷拍丶妓女丶自由| 久久99久久99精品中文字幕| 激情五月色综合国产精品| 精品国模一区二区三区| 有车车的腐肉动画网入口| 中文字幕奈奈美被公侵犯| 999ZYZ玖玖资源站永久无码| 国产精品99久久久久久人四虎| 国产亲妺妺乱的性视频播放| 欧美边做饭边被躁BD在线看| 无码JK粉嫩小泬在线观看欧美| 第一次进去丫头的身体怎么办 | 欧美又黄又嫩大片a级| 高潮AAA人人爽人人爱| 国产在线av一区二区麻豆董小宛| 亚洲熟妇无码一区二区三区导航 | 熟妇人妻AV中文字幕老熟妇| 亚洲AV无码一区二区一二区| 日韩一区二区三区精品| 性视频播放免费视频| 亚洲AV乱码久久精品蜜桃| 成人欧美一区二区三区在线观看| 精品久久久久中文字幕| 国产精品久久无码一区| 诱人的女同学hd中文字幕|