玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CCL-220™COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

簡(jiǎn)要描述:COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CCL-220™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1670

產(chǎn)品分類

Product Category

詳細(xì)介紹

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320DM (ATCC® CCL-220™)

Organism  Homo sapiens, human

Tissue  colon

Product Format  frozen

Morphology  rounded and refractile

Culture Properties  mixed, adherent and suspension

Biosafety Level  1

Disease  Dukes' type C, colorectal adenocarcinoma

Age  55 years

Gender  female

Ethnicity  Caucasian

Storage Conditions

Karyotype  modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Images

Clinical Data  55 years

Caucasian

female

Genes Expressed  serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Tumorigenic  Yes

Effects  Yes, in nude mice

Comments  The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

Subculturing  Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

3.Add appropriate aliquots of the cell suspension to new culture vessels.

4.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation  Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Isoenzymes  ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Name of Depositor  GE Moore

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
狠狠人妻久久久久久综合九色| FREEXXXX性中囯HD性| 国产精品无码久久久久不卡| 亚洲人成色777777精品音频| 亚洲精品一区中文字幕乱码| 极品少妇高潮啪啪AV无码| 欧美人妻精品一区二区三区| 国产AV无遮挡喷水白浆桃花视频| 狠狠色噜噜狠狠狠狠色综合久| 亚洲国产一区二区| 狠狠狠色丁香婷婷综合久久AV| 被绑在机器上榨精失禁男男| 暑假全天自我体罚PG具体方案| 无码欧亚熟妇人妻AV在线| 巨物挺破了校花那层薄膜 | 国产精品第一页| 把极品白丝班长啪到腿软| 日韩人妻精品无码久久上司| 日本添下边无码视频| 国产精品婷婷久久爽一下| 免费看男男GAY啪啪网站| 无人区乱码一区二区三区| 意大利性经典XXXXX| 欧美A级情欲片在线观看免费| 地瓜视频app免费观看下载| 天天做天天爱夜夜爽毛片毛片| 波多野结衣办公室激情A片| 人妻熟人中文字幕一区二区| 97人妻天天摸天天爽天天| 久久伊人蜜桃AV一区二区| 超碰CAOPOREN97人人| 国产97色在线 | 日韩| 337P日本欧洲亚洲大胆张筱雨| 朋友出差人妻半推半就滑进| 1314女人裸体A片| 久久久久久久久久久精品尤物| 国产人妻精品一区二区三区| 又白又嫩毛又多12P| 又硬又粗进去爽A片免费| 精品久久久久久无码中文字幕一区| 鸡毛飞上天电视剧全集观看免费|