玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-9444MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

簡(jiǎn)要描述:MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫(kù)管理規(guī)范,提供 培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-9444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2069

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

ATCC® Number:CRL-9444™    Price:$329.00
Designations:MeT-5A

Depositors:The United States of America

Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Tissue: mesothelium
Cell Type: epithelialvirus transformed


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,13

D16S539: 12

D5S818: 12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 15,18



Comments:Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals.

The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned.

The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc.

The cells stain positively for vimentin, keratins and SV40 T antigen.



Propagation:ATCC complete growth medium: Medium199 with Earle's BSS, .75 mM L-glutamine and 1.25 g/L sodium bicarbonate supplemented with with 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin, 20 mM HEPES, trace elements (see above reference) and 10% fetal bovine serum.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:recommended serum:ATCC 30-2020

References:21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
23292: Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952

























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
风流少妇A片一区二区蜜桃| 色婷婷五月综合中文字幕| 浪货夹的真紧好爽小雪| 亚洲日韩精品AV成人波多野 | 两腿间花蒂被吸得肿了视频| 粗大的内捧猛烈进出在线视频| 一本色道在线久88在线观看片| 99香蕉国产精品偷在线观看| 女浴室里赤裸裸洗澡丰满视频 | 久久精品国产大片免费观看| 国产在线国偷精品免费看| 内谢少妇XXXXX8老少交| 少妇被粗大的猛烈进出免费视频| 末成年女AV片一区二区| 99精品国产在热久久婷婷| 97超级碰碰碰免费公开在线观看| 不断作死后我成了白月光| 国产精品高潮呻吟av久久96| 我把我的肥岳日出水来73| 亚洲国产精品18久久久久久| 大屁股熟女一区二区三区| 又粗又硬又黄A级毛片| 当兵XXXX做受当兵GAY| 亚洲熟妇少妇任你躁在线观看无码 | 麻豆人妻少妇精品无码专区| 被多个男人调教奶头玩奶头| 正在播放老肥熟妇露脸| 娇小性XXXX摘花HD| 久久久久久久做爰片无码| 久久久久亚洲精品男人的天堂| 麻豆妓女爽爽一区二区三| 玉蒲团夜宵魂免费观看| 亚洲乱码爆乳精品成人毛片| 精国产品一区二区三区A片| 久久精品免费一区二区三区 | 人体艺术在线观看| 学长上课揉搓捏掐我奶H| 日韩国产成人无码AV毛片| 丰满人妻在公车被猛烈进入电影 | 妈妈的朋友6| 玩弄老太婆BBW视频|