玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-9444MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

簡(jiǎn)要描述:MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫(kù)管理規(guī)范,提供 培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-9444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:2069

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

ATCC® Number:CRL-9444™    Price:$329.00
Designations:MeT-5A

Depositors:The United States of America

Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Tissue: mesothelium
Cell Type: epithelialvirus transformed


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MeT-5A細(xì)胞, 人類(lèi)肋膜間皮細(xì)胞

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,13

D16S539: 12

D5S818: 12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 15,18



Comments:Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals.

The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned.

The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc.

The cells stain positively for vimentin, keratins and SV40 T antigen.



Propagation:ATCC complete growth medium: Medium199 with Earle's BSS, .75 mM L-glutamine and 1.25 g/L sodium bicarbonate supplemented with with 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin, 20 mM HEPES, trace elements (see above reference) and 10% fetal bovine serum.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:recommended serum:ATCC 30-2020

References:21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
23292: Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952

























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
黑人双人RAPPER| 日日碰狠狠添天天爽超碰97| 免费人成视频在线观看| 交换俱乐部娇妻奶呻吟啊视频 | 国产全黄三级| 末发育女av片一区二区在线观看| 国产SUV精品一区二区88L| 把舌头伸出来呼吸舒服一点| 欧美亚洲一区二区三区| 久久AV高潮AV无码AV喷吹| 最近2019中文字幕大全视频1| 奇米777四色影视在线看| 久久久久亚洲AV片无码下载蜜桃| 日本巨大超乳爆乳| 性高湖久久久久久久久AAAAA | proxxon是国产| 久久人妻少妇嫩草AV蜜桃| 国产寡妇XXXX猛交| 亚洲精品乱码久久久久久自慰| 无码人妻一区二区三区在线| 国产AV日韩A∨亚洲AV电影| 午夜精品一区二区三区免费视频| 乱人伦精品视频在线观看| 久久亚洲熟女cc98cm| 少妇与公做了一夜伦理| 亚洲精品在线观看| 无码少妇高潮浪潮AV久久| 局长含着娇妻的一对高耸| 久久久久久久97| 亚洲精品粉嫩小泬18P| 国产日产亚洲系列最新| 欧美精品一区二区少妇免费A片| 成熟YIN荡美妞A片视频麻豆| 免费高清A级毛片在线播放 | 国产精品久久久久精品三级卜| 女人被狂C躁到高潮视频免费网站| 色婷婷国产精品视频一区二区三区| 久久久久噜噜噜亚洲熟女综合| 精品久久久噜噜噜久久久| 精品亚洲成A人在线观看青青| 精品一区二区三区|