玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-11610RWPE-2 人前列腺正常細(xì)胞

RWPE-2 人前列腺正常細(xì)胞

簡(jiǎn)要描述:CRL-11610 RWPE-2 人前列腺正常細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-11610
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2959

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-11610 RWPE-2 人前列腺正常細(xì)胞

ATCC® Number:CRL-11610™    Price:
Designations:RWPE-2

Depositors:Michigan State University, National Cancer Institute

Biosafety Level:2 [Cells contain Human Papilloma viral (HPV) sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:CRL-11610 RWPE-2 人前列腺正常細(xì)胞epithelial


Source:Organ: prostate
Disease: normal
Cell Type: epithelial


Cellular Products:cytokeratin 18 [46793]

cytokeratin 8 [46793]



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Receptors:androgen receptor, expressed ( [46793] upregulated upon exposure to androgen)

Tumorigenic:Yes

Antigen Expression:kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) ( [46793] upon exposure to androgen)

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8,9.3

TPOX: 8,11

vWA: 14,18



Cytogenetic Analysis:At passage 14, a majority of the cells were in the near diploid range (48-54), with the main population being 51, XY. [46795]

Isoenzymes:AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 0

PGM1, 2

PGM3, 1



Gender:CRL-11610 male

Ethnicity:Caucasian, White

Comments:Tumor Suppressor Gene(s):pRB + [PubMed: 9214605]

RWPE-2 cells were derived from RWPE-1 cells (ATCC CRL-11609) by transformation with Ki-ras using the Kirsten murine sarcoma virus (Ki-MuSV) [PubMed: 9214605]. Epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome to establish the RWPE-1 cell line (ATCC CRL-11609). RWPE-1 cells neither grow in agar nor form tumors when injected into nude mice with or without Matrigel. However, RWPE-2 cells form small colonies in soft agar and form tumors when injected into nude mice [PubMed: 9214605]. Further, a family of tumorigenic cell lines, that mimics multiple steps in prostate cancer progression, was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrsourea (MNU). See the WPE1-NA22 (ATCCCRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell ines.

The depositor reports that the RWPE-1 cell line (ATCC CRL-11609) was screened, and found negative for, Hepatitis B virus, Hepatitis C virus and Human immunodeficiency virus.



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C



Subculturing:CRL-11610 Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).

  3. Add 2.0 to 3.0 ml (to a T-25 flask) or 3.0 to 4.0 ml (to a T-75 flask) of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.

  4. Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS), as appropriate, and aspirate cells by gently pipetting.

  5. Transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 7 minutes.

  6. Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 4 X 10(4) and 8 X 10(4) cells/sq. cm.

Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 days


Preservation:Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:parental cell line:ATCC CRL-11609

purified DNA:ATCC CRL-11610D

derived from same individual:ATCC CRL-2849

derived from same individual:ATCC CRL-2850

derived from same individual:ATCC CRL-2852

derived from same individual:ATCC CRL-2851

derivative:ATCC CRL-2853

derived from same individual:ATCC CRL-2854



References:46792: Webber MM, Rhim JS. Immortalized and malignant human prostatic cell lines. US Patent 5,824,488 dated Oct 20 1998
46793: Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
53180: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636
53181: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337
53182: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152
53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759
53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497
CRL-11610


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产99久久久国产精品潘金莲| 无码国内精品人妻少妇蜜桃视频| 欧美重囗味sm群虐视频| 久久中文字幕人妻熟av女蜜柚m| 国内精品视频一区二区三区八戒| 国产精品一区二区| 粗大的内捧猛烈进出| 欧洲亚洲精品a片久久99果冻| 最近日本韩国高清免费观看| 锕锕锕锕锕锕~再深一点五种软件 | 极品少妇高潮啪啪av无码吴梦梦 | 欧美激情性做爰免费视频| 白丝袜小舞被撕开裆部图片| 午夜精品久久久久久中宇 | 欧洲熟妇色XXXX欧美老妇多毛 | 有哪些黄色网站| 国产免费无码又爽又刺激A片| 国产午夜福利100集发布| 丰满爆乳无码一区二区三区| 40厘米黑人与中国女人| 日本精品久久久久中文字幕| 国产精品 视频一区 二区三区| 欧美,日韩,国产,专区| 最近2019中文字幕大全第二页 | 亚洲午夜久久久久久久久电影网| 国产无套白浆一区二区| 无码亚洲成A∧人片在线播放| 五十路六十路老熟妇A片| 出差被夫の上司持久侵犯三浦步美| 日本熟妇乱妇熟色A片蜜桃| 国产+成+人+亚洲欧洲自| 免费特级毛片| 日本少女漫画| 你是我的城池营垒全集免费观看 | 女学生被躁到高潮免费视频| 午夜无码熟熟妇丰满人妻 | 老女人重囗味456| 12—13女人做A片| 五月天人体艺术| 日本XXXB孕妇孕交视频| 学生姝被内谢出白浆|